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length of microscope formula. It is a convex lens of short focal length mounted in a lens holder. To obtain magnification it is placed at a small distance from the object and virtual image is formed.
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Record the distance in mm and µm. N is the refractive index l is the wavelength NA is the Numerical Aperture and f is the focal length. And eyepiece focal length f e mm.
N is the refractive index l is the wavelength NA is the Numerical Aperture and f is the focal length.
Therefore magnification will be m 1 Df Since D is about 25 cm to have a magnification of six one needs a convex lens of focal length f 5 cm. Typically the tube lens is an achromatic lens with a focal length of 200mm but other focal lengths can be substituted as well thereby customizing a microscope systems total magnification. Presented in Figure 1 is a schematic illustration of an objective showing the parfocal and working distance length specifications as well as other descriptions engraved or printed on the objective barrel. In general the objective working distance decreases as the magnification and numerical aperture both increase as presented in Table 1 for a highly corrected series of Nikon plan fluorite.
