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how to calculate log fold change in r. For calculating Fold change from log2 just do Power 2 log2_Value Power 2 0584915. CT value Baseline condition Housekeeping gene.
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For calculating Fold change from log2 just do Power 2 log2_Value Power 2 0584915. You need to calculate the value of 2-DDCt to get the expression fold change. Then put the equation in Excel Log FC 2 to get the log2.
However now I would like to calculate a p-value for the identified fold changes if possible.
If the base is specified log xb computes logarithms with base b. It seems like Limmas LogFC is actual fold change just involved log2-transformed values. First you have to divide the FPKM of the second value of the second group on the FPKM of the first value to get the Fold Change FC. I think youre right about Limmas calculation.
