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how to calculate log fold change. And Ct Exp-Control and got the -Ct log-fold-change. You would only do a t-test between controltreated if you want to test for difference in the sample means but not for calculating the fold-change.
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I am trying to find a good solution on how to calculate fold change for qPCR data normalised to housekeepting genes across different conditions. For all genes scored the fold change was calculated by dividing the mutant value by the wild type value. Fold change is calculated simply as the ratio of the difference between final value and the initial value over the original value.
There is an alternative definition of fold change citation needed although this has generally fallen out of use.
Fold change is typically calculated by simply average of group 2 average of group 1. For quantities A and B the fold change is given as B AA or equivalently BA 1. 307 306 306 Baseline condition Gene X. 075 or a drop of 25 from wild type is reported as either 13 fold down or -13 fold change.
