Managed or edit the displayed columns by right. Click New and define the columns title. This means that all SEQ and ACK numbers always start at 0 for the first packet seen in each conversation The picture below shows a real example of TCP sequence and acknowledgment numbers in a TCP flow diagram.
Wireshark already does this calculation for you.
We add the amount of data sent to the current sequence number then we get the next expected sequence number 101. Right click on that and hit Apply as Column Reply. Expand the TCP section of the packet details and look for Next sequence number. This means that all SEQ and ACK numbers always start at 0 for the first packet seen in each conversation The picture below shows a real example of TCP sequence and acknowledgment numbers in a TCP flow diagram.