If your CFU is 25 your aliquot is 1cc and your dilution factor is 10 - 2 the calculation of the log CFU mL is. 8 ml of Bacterial Culture is added to 53 ml of sterile diluent. Hence you need calibration curves for CFUml at each cellsml value and cellsml at each OD value.
Yes you can calculate using the formula cfuml no.
Of colonies x dilution factor volume of culture plate. You must do this to. Divide the CFU from the dilution 179 by the result from Step 1 0005 to yield 35800 CFU. So total colony forming unit 15 x 108 per mL Now we may want to convert the values to Log value.